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Laboratory Technician in Structural Cell Biology (15 hours per week)

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Laboratory Technician position (15 hours per week) in Structural Cell Biology at the Protein Structure & Function Program at the Novo Nordisk Foundation Center for Protein Research

Tenure: permanent.

Presentation of the Center The Novo Nordisk Foundation Center for Protein Research (CPR, www.cpr.ku.dk ) at the Faculty of Health and Medical Sciences, University of Copenhagen, performs basic and applied discovery research on human proteins of biomedical relevance. The Center comprises a wide range of expertise and resources, from in silico target identification, mass spectrometry based proteomics, protein production and characterization, to elucidation of various disease mechanisms.

The Group headed by Guillermo Montoya focuses in macromolecular complexes. These assemblies underlie all biological processes and play either dynamic roles in catalysis or signaling or static roles in scaffolding or information storage in the cell. The focus of our group is the molecular understanding of the role played by assemblies of these macromolecules involved in cell cycle and genome integrity related processes.

Job description

  • To perform laboratory work within the areas of sub-cloning, cell culture, recombinant expression, protein purification and protein crystallization of medically relevant proteins.
  • To purify recombinant proteins at the mg-scale using various types of column chromatography, such as affinity chromatography, ion-exchange, hydrophobic interaction chromatography, reversed phase, and size exclusion.
  • Techniques used will include ligation independent cloning and other cloning methods, expression of recombinant proteins from prokaryotic hosts (primarily E. coli), liquid chromatography, SDS-PAGE and Western blotting.
  • To evaluate and report experimental results using Electronic Laboratory Notebooks and Laboratory Information Management Systems established at the center.
  • To maintain stocks, reagents, media and laboratory equipment.
  • To share responsibility within the team in terms of defining objectives and meet pre-set deadlines.
  • To participate in the general operation and maintenance of the laboratory facilities.
  • To carry out any other relevant duties as may reasonably be associated with the position and which may be required from time to time.

Experience and skills

  • Proven experience in molecular biology, prokaryotic recombinant protein expression and protein purification and characterization. Previous experience with automated liquid handling system would be considered an asset.
  • Computer proficiency (Word, Excel, Powerpoint). Experience with DNA cloning and sequencing software and research database systems would be considered merits.
  • Good communication skills, both oral and written. Considering that the Center is a highly international research environment, good knowledge in English is required.
  • Integrity, enthusiasm, motivation, flexibility, confidence and good collaboration skills.

Qualifications

Laboratory technician degree or equivalent is required. Solid and documented work experience from high quality academic and/or industrial laboratories is required. The ability to efficiently work as part of a team is essential.

Place of employment and work

Employment is at CPR (www.cpr.ku.dk) University of Copenhagen, located in central Copenhagen. The research environment at CPR is vibrant, ambitious, and international, with approx. 30 different nationalities currently represented. The Center comprises modern laboratories and state-of-the-art facilities within protein science.

Terms of salary and employment

Employment will be in accordance with the provisions of the collective agreement between the Danish Government and HK-STAT (Danish Technician Association). The position will be at the level of salary group 4 with the possibility to negotiate a salary supplement and the working hours are 15 hours per week. To this is added a monthly contribution to a pension fund according to the collective agreement. In all cases, ability to perform the job will be the primary consideration, and thus we encourage all – regardless of their personal background and status - to apply. The position is permanent.

Tentative start date: November 2015

Application
Your application must be submitted electronically by clicking ‘APPLY NOW’ below or via this advertisement found on http://employment.ku.dk/.

Deadline for application: 25 October 2015

For further information about the position, please contact CPR: Jobs@cpr.ku.dk

EOJD